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- Carbohydrate Analysis bf8 (1 MB )
- Carbohydrate Analysis bf8 (1 MB )
- L2879 - Enzyme Assay (12 KB)
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L2879
Sigma
Lysozyme chloride form from chicken egg white
Grade VI, ≥45,000 units/mg protein (E1%/282 )
| Synonym: | Mucopeptide N-acetylmuramoylhydrolase, Muramidase |
| CAS Number: | 9066-59-5 |
| Enzyme Commission (EC) Number: | 3.2.1.17 ( BRENDA | IUBMB ) |
| EC Number: | 232-954-0 |
| MDL number: | MFCD00131557 |
Description
| Application | Enzyme which breaks down the cell walls of bacteria; used to prepare spheroplasts. |
| Preparation Note | 3× Crystallized |
| Unit Definition | One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25°C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path). |
| Physical form | Lyophilized powder containing sodium chloride and sodium acetate |
Properties
| type | Grade VI |
| mol wt | mol wt ~14.3 kDa |
| composition | Protein, ≥80% |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 2 |
| RTECS | OL5989850 |
